dose-response – inhibition log(inhibitor) vs. response – variable slope equation in Search Results


celltiter 96® aqueous one solution cell proliferation assay  (Promega)
90
Promega celltiter 96® aqueous one solution cell proliferation assay
Celltiter 96® Aqueous One Solution Cell Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
celltiter 96® aqueous one solution cell proliferation assay - by Bioz Stars, 2026-10
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automated system  (ImStar Therapeutics)
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ImStar Therapeutics automated system
Automated System, supplied by ImStar Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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celltiter-glo assay  (Promega)
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Promega celltiter-glo assay
Celltiter Glo Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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molport-137  (MolPort Inc)
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MolPort Inc molport-137
Molport 137, supplied by MolPort Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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origin software  (OriginLab corp)
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OriginLab corp origin software
Origin Software, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
origin software - by Bioz Stars, 2026-10
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ahu377  (Novartis)
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Novartis ahu377
Ahu377, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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temperature-related intensity (tric  (NanoTemper Technologies)
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NanoTemper Technologies temperature-related intensity (tric
(A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the <t>binding</t> <t>affinity</t> analysis, ligand-dependent changes in temperature-related intensity change <t>(TRIC)</t> https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).
Temperature Related Intensity (Tric, supplied by NanoTemper Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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temperature-related intensity (tric - by Bioz Stars, 2026-10
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quest graphtm ec50 calculator  (AAT Bioquest)
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AAT Bioquest quest graphtm ec50 calculator
(A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the <t>binding</t> <t>affinity</t> analysis, ligand-dependent changes in temperature-related intensity change <t>(TRIC)</t> https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).
Quest Graphtm Ec50 Calculator, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quest graphtm ec50 calculator - by Bioz Stars, 2026-10
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from painful tumor 1  (CH Instruments)
90
CH Instruments from painful tumor 1
(A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the <t>binding</t> <t>affinity</t> analysis, ligand-dependent changes in temperature-related intensity change <t>(TRIC)</t> https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).
From Painful Tumor 1, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dose-response+%E2%80%93+inhibition+log(inhibitor)+vs%2E+response+%E2%80%93+variable+slope+equation+in/from+painful+tumor+1/pmc06739480-80-15-47
Average 90 stars, based on 1 article reviews
from painful tumor 1 - by Bioz Stars, 2026-10
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atogepant  (Allergan)
86
Allergan atogepant
(A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the <t>binding</t> <t>affinity</t> analysis, ligand-dependent changes in temperature-related intensity change <t>(TRIC)</t> https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).
Atogepant, supplied by Allergan, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dose-response+%E2%80%93+inhibition+log(inhibitor)+vs%2E+response+%E2%80%93+variable+slope+equation+in/atogepant/fda_document____drugsatfda_docs_slash_nda_slash_2021_slash_215206orig1s000integratedr-2371-28-30
Average 86 stars, based on 1 article reviews
atogepant - by Bioz Stars, 2026-10
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parallel line assay version 2.0  (Stegmann Systems GmbH)
90
Stegmann Systems GmbH parallel line assay version 2.0
(A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the <t>binding</t> <t>affinity</t> analysis, ligand-dependent changes in temperature-related intensity change <t>(TRIC)</t> https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).
Parallel Line Assay Version 2.0, supplied by Stegmann Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
parallel line assay version 2.0 - by Bioz Stars, 2026-10
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mdco-2010  (The Medicines Company)
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The Medicines Company mdco-2010
(A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the <t>binding</t> <t>affinity</t> analysis, ligand-dependent changes in temperature-related intensity change <t>(TRIC)</t> https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).
Mdco 2010, supplied by The Medicines Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mdco-2010 - by Bioz Stars, 2026-10
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Image Search Results


(A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the binding affinity analysis, ligand-dependent changes in temperature-related intensity change (TRIC) https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).

Journal: PLOS Pathogens

Article Title: Withaferin A inhibits Chikungunya virus nsP2 protease and shows antiviral activity in the cell culture and mouse model of virus infection

doi: 10.1371/journal.ppat.1012816

Figure Lengend Snippet: (A) Microscale thermophoresis was used to study the binding of the labelled CHIKV nsP2 protein (1 μM) with different concentrations of WFA or Wn. The bar graphs display the fluorescence change (ΔF) obtained on nsP2 binding with different concentrations of WFA or Wn in reference to the unbound protein state in the buffer. The left panel shows the nsP2 binding with different WFA concentrations. The right panel shows nsP2 binding with WFA and Wn at the indicated concentration. The student’s t-test was used to calculate the p value; *** p <0.001. (B) To examine the binding and determine the equilibrium dissociation constant between CHIKV nsP2, its protease and helicase domains, and WFA, 1 μM labelled protein was titrated with different concentrations of WFA ranging from 250 to 0.03 μM. (C) To study the effect of the nsP2 mutation on WFA binding 1 μM labelled protein was titrated with different concentrations of WFA. For the binding affinity analysis, ligand-dependent changes in temperature-related intensity change (TRIC) https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values against the WFA concentrations in a dose-response curve. The F norm values are plotted as parts per thousand (‰).

Article Snippet: For the binding affinity analysis, the ligand-dependent changes in the temperature-related intensity change (TRIC) https://nanotempertech.com/nanopedia/tric/ are plotted as F norm values vs . ligand concentration in a dose-response curve.

Techniques: Microscale Thermophoresis, Binding Assay, Fluorescence, Concentration Assay, Mutagenesis